Table 1 - Basal Rates

Trial #	Temperature °C	Breaths/minute
1	19	60
2	20	70
3	21	80
Averages	20	70

Hypothesis: If temperature increases, then breathing rate increases.

Table 2 - Experimental Data

Condition	Experimental	Breaths/minute
	Temperature °C	Trial 1	Trial 2	Trial 3	Average
1. cold	10	15	20	25	20
1.5. medium	15	48	50	52	50
2. room	20	60	70	80	70
3. warm	30	90	100	110	100

GRAPH AND CONCLUSION p. 188

Graph

Conclusion: Breathing rate (and metabolic rate) in goldfish increases with temperature.

QUESTIONS

1. List at least 2 factors that you kept constant in the experiment.
   Keep fish calm (same activity level), same size beaker, same amount of water.

2. Explain why you brought the temperature back to the basal temperature before returning the fish to the tank.
   Avoid temperature shock to the fish.

3. Was your hypothesis supported by your data? Explain why or why not using the data to support your answer.
   Hopefully breathing rate increased with temperature, without cooking fish.

4. How does the increased water temperature affect the breathing movements of the fish?
   Increased water temperature leads to faster metabolism and faster breathing.

   How does this affect the amount of oxygen required for metabolism?
   More oxygen is needed for cellular respiration to support faster metabolism.

5. What other factor causes the oxygen supply to be used up more rapidly in warm water than in cold water?
   The amount of dissolved oxygen decreases with higher water temperature.

6. If an aquatic organism required a lot of oxygen, would you expect to find it living in cold or warm water?
   Cold, because cold water contains more dissolved oxygen.

7. If heated water entered a river, what effects do you predict the thermal pollution would have on the organisms?
   Fewer cold-adapted organisms, such as sunfish and bluegill) would survive; more warm-adapted organisms, such as goldfish and carp, may take over.